Diagnóstico serológico y molecular del Potato leafroll virus (PLRV) en tubérculos-semilla de papa en Antioquia, Colombia
نویسندگان
چکیده
منابع مشابه
Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk
RESUMEN Potato virus S (PVS) is a prevalent virus in potato fields in Colombia and the rest of the world. PVS has been classified into two separate lineages, PVS (Ordinary) and PVS (Andean), which are genetically distinct. In this study, the complete genome sequence of a new PVS isolate (PVS_Antioquia) was obtained using High-throughput sequencing (Illumina HiSeq-2000) from tuber sprouts of Sol...
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RESUMEN Potato virus Y (PVY) is one of the most severe viruses affecting the production of potato (Solanum tuberosum) in the world. This study presents a detailed molecular analysis using nextgeneration sequencing (NGS), IC-RT-qPCR and RT-PCR on the PVY isolates infecting seed-tubers and foliage of potato plants cv. Diacol-Capiro in La Union (Antioquia, Colombia). Analysis of incidence by IC-RT...
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متن کاملDigoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV).
A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUC18 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT prob...
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ژورنال
عنوان ژورنال: Acta Agronómica
سال: 2015
ISSN: 2323-0118,0120-2812
DOI: 10.15446/acag.v65n2.50764